Journal: Frontiers in Pharmacology
Article Title: Induction of apoptosis by ethanol extract of sapodilla leaf in HGT-1 cells involved caspase-independent signal transduction pathway and activation of Nrf2/HO-1
doi: 10.3389/fphar.2026.1803513
Figure Lengend Snippet: Effect of sapodilla leaf 70% ethanol extract on cancer and non-cancerous cell lines. (A) The cell viability after 24, 48, and 72 h exposure with sapodilla leaf 70% ethanol extract on human prostate cancer (PC-3) cell line using MTT assay. (B) sapodilla leaf 70% ethanol extract increases the viability of PC-3 cell line after 24, 48, and 72 h evaluated using LDH assay. (C) The cell viability of mouse fibroblast (BALB/c 3T3) cell line evaluated by MTT assay. (D) Treatment of sapodilla leaf 70% ethanol extract in BALB/c 3T3 cells using LDH assay. The data are presented as mean ± SD (n = 3). The data with different superscript letter indicates a significant difference between groups by Tukey’s test ( P < 0.05).
Article Snippet: A panel of cell lines comprising mouse fibroblast (BALB/c 3T3), human prostate carcinoma (PC-3), cervical cancer (HeLa), gastric carcinoma (HGT-1), colorectal adenocarcinoma (HT-29), colon carcinoma (HCT-116), and hepatocellular carcinoma (HepG2) was utilized in this study, all of which were sourced from the American Type Culture Collection (ATCC).
Techniques: MTT Assay, Lactate Dehydrogenase Assay